Second Bio-Blog March 5, 2010
Posted by RhyanVillanueva in Uncategorized.trackback
Hi, again!!!!
In the previous laboratory meeting, we had training on how to use a digital multichannel pipette. This is a powerful laboratory technique that will reduce systematic errors during the pippeting process and that will save a lot of time. I have always believed that there is only one technique for pippeting. But in the training, I learned that there is another type of technique called reverse pippeting that I can use for more viscous liquids and/or low volume discharge.
Actually, I am working actively on the laboratory. However, I have been on the laboratory for about only four weeks, in which I have attended several laboratory meetings and I am constantly learning new techniques in addition to information. I have to tell that I am not been able to achieve the proposed semester goals, which indicate a 1 on the provided scale. It is important to note that I am actively working and learning for the completion of the specified goals.
In the laboratory, there are many sub-pools (samples) that were taken from the Yunque & Guanica forests and contain metagenomic DNA. Answering the question, about the difficulties that I am having; is that there are many samples to look for Dnase activity. But this is very positive, because there are greater chances for finding a colony with Dnase activity.
Thanks for your interest!
Es importante que compartas los objetivos que persigues en el lab. Dejanos saber si tienes planes de investigacion para el verano.
Hi, Dr. Buxeda!
The objectives are the following (extracted from the first post):
The first objective is to isolate the responsible gene and protein for expressing Dnase activity. Another is to characterize the gene and its product. Last but not least, to sequence the gene, and to characterize the protein structurally and kinetically.
Actually, I applied to several universities in the US. Still, have not received a final answer. Looking forward to it! Thanks for asking.
Rhyan, it is good to know you could find another project. So far there are many things to learn, I hope you will acquire a complete set of research skills that you’ll be able to extrapolate in the future.
L. Saliceti
Thanks, Dr. Saliceti! I am actually learning a lot into areas that I did not have any knowledge at all! I really love it!!!
Good luck in your research, I really hope you will be able to reach all of your goals.
Thanks, Natalia. I really appreciate it! I wish more great things to you!